Designed divergent evolution of enzyme function

@article{Yoshikuni2006DesignedDE,
  title={Designed divergent evolution of enzyme function},
  author={Yasuo Yoshikuni and Thomas E. Ferrin and Jay D. Keasling},
  journal={Nature},
  year={2006},
  volume={440},
  pages={1078-1082}
}
It is generally believed that proteins with promiscuous functions divergently evolved to acquire higher specificity and activity, and that this process was highly dependent on the ability of proteins to alter their functions with a small number of amino acid substitutions (plasticity). The application of this theory of divergent molecular evolution to promiscuous enzymes may allow us to design enzymes with more specificity and higher activity. Many structural and biochemical analyses have… Expand
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