Designed ankyrin repeat proteins (DARPins) from research to therapy.

@article{Tamaskovic2012DesignedAR,
  title={Designed ankyrin repeat proteins (DARPins) from research to therapy.},
  author={R. Tamaskovic and M. Simon and Nikolas Stefan and Martin Schwill and A. Pl{\"u}ckthun},
  journal={Methods in enzymology},
  year={2012},
  volume={503},
  pages={
          101-34
        }
}
Designed ankyrin repeat proteins (DARPins) have been developed into a robust and versatile scaffold for binding proteins. High-affinity binders are routinely selected by ribosome display and phage display. DARPins have entered clinical trials and have found numerous uses in research, due to their high stability and robust folding, allowing many new molecular formats. We summarize the DARPin properties and highlight some biomedical applications. Protocols are given for labeling with dyes and… Expand
Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
  • A. Plückthun
  • Biology, Medicine
  • Annual review of pharmacology and toxicology
  • 2015
Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs.
odeling of in Repeat Protein Their Targets eas Plückthun
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