Design of the linkers which effectively separate domains of a bifunctional fusion protein.

@article{Arai2001DesignOT,
  title={Design of the linkers which effectively separate domains of a bifunctional fusion protein.},
  author={Ryoichi Arai and Hiroshi Ueda and Atsushi Kitayama and Nozomi Kamiya and Teruyuki Nagamune},
  journal={Protein engineering},
  year={2001},
  volume={14 8},
  pages={
          529-32
        }
}
With the aim of separating the domains of a bifunctional fusion protein, the ability of several lengths of helix-forming peptides to separate two weakly interacting beta-can domains was compared with that of flexible linkers or of a three alpha-helices bundle domain. We introduced helix-forming peptide linkers A(EAAAK)nA (n = 2-5) between two green fluorescent protein variants, EBFP and EGFP, and investigated their spectral properties. The fluorescence resonance energy transfer from EBFP to… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 168 CITATIONS, ESTIMATED 69% COVERAGE

Regulated offloading of cytoplasmic dynein from microtubule plus ends to the cortex.

  • Developmental cell
  • 2011
VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Construction of a linker library with widely controllable flexibility for fusion protein design

  • Applied Microbiology and Biotechnology
  • 2015
VIEW 9 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Assembling a xylanase-lichenase chimera through all-atom molecular dynamics simulations.

  • Biochimica et biophysica acta
  • 2013
VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Improved Production of Highly Active and Pure Human Creatine Kinase MB.

  • Journal of molecular microbiology and biotechnology
  • 2018
VIEW 3 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

Development of plant-produced Bluetongue virus vaccines

VIEW 2 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2003
2019

CITATION STATISTICS

  • 12 Highly Influenced Citations

  • Averaged 17 Citations per year from 2017 through 2019