Design of multivalent complexes using the barnase·barstar module

@article{Deyev2003DesignOM,
  title={Design of multivalent complexes using the barnase·barstar module},
  author={Sergey M. Deyev and Robert J Waibel and Ekaterina N. Lebedenko and August P. Schubiger and Andreas Pl{\"u}ckthun},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={1486-1492}
}
The ribonuclease barnase (12 kDa) and its inhibitor barstar (10 kDa) form a very tight complex in which all N and C termini are accessible for fusion. Here we exploit this system to create modular targeting molecules based on antibody scFv fragment fusions to barnase, to two barnase molecules in series and to barstar. We describe the construction, production and purification of defined dimeric and trimeric complexes. Immobilized barnase fusions are used to capture barstar fusions from crude… CONTINUE READING

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