Design of metabolic engineering strategies for maximizing L-(-)-carnitine production by Escherichia coli. Integration of the metabolic and bioreactor levels.

@article{Sevilla2005DesignOM,
  title={Design of metabolic engineering strategies for maximizing L-(-)-carnitine production by Escherichia coli. Integration of the metabolic and bioreactor levels.},
  author={{\'A}ngel Sevilla and Julio Vera and Zuleyka D{\'i}az and M C{\'a}novas and N{\'e}stor V. Torres and Jos{\'e} Luis Iborra},
  journal={Biotechnology progress},
  year={2005},
  volume={21 2},
  pages={329-37}
}
In this work metabolic engineering strategies for maximizing L-(-)-carnitine production by Escherichia coli based on the Biochemical System Theory and the Indirect Optimization Method are presented. The model integrates the metabolic and the bioreactor levels using power-law formalism. Based on the S-system model, the Indirect Optimization Method was applied, leading to profiles of parameter values that are compatible with both the physiology of the cells and the bioreactor system operating… CONTINUE READING