Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups.

@article{Wright2014DesignOM,
  title={Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups.},
  author={Emma J Wright and Maciej Sosna and Sally Bloodworth and Jeremy D Kilburn and Philip N Bartlett},
  journal={Chemistry},
  year={2014},
  volume={20 19},
  pages={5550-4}
}
Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (≈7.5… CONTINUE READING