Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution.

@article{Doi1999DesignOG,
  title={Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution.},
  author={Naoko Doi and Hiroshi Yanagawa},
  journal={FEBS letters},
  year={1999},
  volume={453 3},
  pages={
          305-7
        }
}
Protein-engineering techniques have been adapted for the molecular design of biosensors that combine a molecular-recognition site with a signal-transduction function. The optical signal-transduction mechanism of green fluorescent protein (GFP) is most attractive, but hard to combine with a ligand-binding site. Here we describe a general method of creating entirely new molecular-recognition sites on GFPs. At the first step, a protein domain containing a desired molecular-binding site is inserted… CONTINUE READING

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