Design of an optimized scaffold for affibody molecules.

  title={Design of an optimized scaffold for affibody molecules.},
  author={Joachim Feldwisch and Vladimir Tolmachev and Christofer Lendel and Nina Herne and A. -K. Sj{\"o}berg and Barbro Larsson and Daniel Rosik and Eva Lindqvist and Gunilla Fant and Ingmarie H{\"o}id{\'e}n-Guthenberg and Joakim Galli and Per Jonasson and Lars Bertil Abrahms{\'e}n},
  journal={Journal of molecular biology},
  volume={398 2},
Affibody molecules are non-immunoglobulin-derived affinity proteins based on a three-helical bundle protein domain. Here, we describe the design process of an optimized Affibody molecule scaffold with improved properties and a surface distinctly different from that of the parental scaffold. The improvement was achieved by applying an iterative process of amino acid substitutions in the context of the human epidermal growth factor receptor 2 (HER2)-specific Affibody molecule Z(HER2:342… CONTINUE READING