Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis.

@article{Chen2015DesignOA,
  title={Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis.},
  author={Wei Chen and Shan Yu Zhang and Peixia Jiang and Jun Yao and Yongzhi He and Lincai Chen and Xiwu Gui and Zhi-yang Dong and Shuang-Yan Tang},
  journal={Metabolic engineering},
  year={2015},
  volume={30},
  pages={149-155}
}
Advanced high-throughput screening methods for small molecules may have important applications in the metabolic engineering of the biosynthetic pathways of these molecules. Ectoine is an excellent osmoprotectant that has been widely used in cosmetics. In this study, the Escherichia coli regulatory protein AraC was engineered to recognize ectoine as its non-natural effector and to activate transcription upon ectoine binding. As an endogenous reporter of ectoine, the mutated AraC protein was… CONTINUE READING