Design of a Novel Globular Protein Fold with Atomic-Level Accuracy

@article{Kuhlman2003DesignOA,
  title={Design of a Novel Globular Protein Fold with Atomic-Level Accuracy},
  author={Brian Kuhlman and Gautam Dantas and Gregory C. Ireton and Gabriele Varani and Barry L. Stoddard and David Baker},
  journal={Science},
  year={2003},
  volume={302},
  pages={1364 - 1368}
}
A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue α/β protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to… 
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1,491 Citations

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