Design of a Novel Globular Protein Fold with Atomic-Level Accuracy

  title={Design of a Novel Globular Protein Fold with Atomic-Level Accuracy},
  author={B. Kuhlman and G. Dantas and Gregory C. Ireton and G. Varani and B. Stoddard and D. Baker},
  pages={1364 - 1368}
  • B. Kuhlman, G. Dantas, +3 authors D. Baker
  • Published 2003
  • Physics, Medicine
  • Science
  • A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue α/β protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to… CONTINUE READING
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