Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification

Abstract

Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in which one reactant of an exothermic reaction is a liquid-gas phase-change material, thereby eliminating the need for a separate phase-change compartment. This design offers potentially enhanced performance and energy density compared to other chemical and electric heaters.

DOI: 10.1371/journal.pone.0139449

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Cite this paper

@inproceedings{Shah2015DesignOA, title={Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification}, author={Kamal G Shah and Dylan Guelig and Steven P. Diesburg and Joshua R. Buser and Robert Burton and Paul LaBarre and Rebecca R. Richards-Kortum and Bernhard Weigl and Mark Isalan}, booktitle={PloS one}, year={2015} }