Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1

@article{JakutyteGiraitiene2016DesignOA,
  title={Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1},
  author={Lina Jakutyte-Giraitiene and Giedrius Gasiunas},
  journal={Journal of Industrial Microbiology \& Biotechnology},
  year={2016},
  volume={43},
  pages={1183-1188}
}
Clustered regularly interspaced short palindromic repeats (CRISPR) together with CRISPR-associated (cas) genes form an adaptive prokaryotic immune system which provides acquired resistance against viruses and plasmids. Bacillus subtilis presently is the best-characterized laboratory model for Gram-positive bacteria and also widely used for industrial production of enzymes, vitamins and antibiotics. In this study, we show that type II-A CRISPR-Cas system from Streptococcus thermophilus can be… 

Figures and Tables from this paper

CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis
TLDR
This work used a flexible, co-transformation approach where the single guide RNA is inserted in a plasmid for Cas9 co-expression, and the donor DNA is supplied as a linear PCR product observing an editing efficiency of 76%.
Bacteriophage-resistant industrial fermentation strains: from the cradle to CRISPR/Cas9
  • R. H. Baltz
  • Biology, Engineering
    Journal of Industrial Microbiology & Biotechnology
  • 2018
TLDR
CRISPR/Cas9 technology was applied to block future contamination by targeting seven different phage genes for double-strand cleavage and can be applied to many current and future microorganisms used in the biotechnology industry.
Characterization of a novel lytic bacteriophage from an industrial Escherichia coli fermentation process and elimination of virulence using a heterologous CRISPR–Cas9 system
  • M. Halter, J. Zahn
  • Biology
    Journal of Industrial Microbiology & Biotechnology
  • 2018
TLDR
The results indicate that the heterologous bacteriophage-resistance system described herein is useful in eliminating lytic infections of bacter iophage DTL, which was prevalent in environment surrounding the manufacturing facility.
New CRISPR‐Cas9 vectors for genetic modifications of Bacillus species
TLDR
New CRISPR‐Cas vectors for genetic modifications in bacillary species are described, which are highly efficient (up to 97%) and precise and should be functional in a broad host range.
CRISPR technologies for bacterial systems: Current achievements and future directions.
The CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis.
TLDR
All the important additions made to the B. subtilis CRISPR toolbox since 2016 are covered, and strain developments fomented by the technology are covered.
Expanding and understanding the CRISPR toolbox for Bacillus subtilis with MAD7 and dMAD7
TLDR
The first use of MAD7 for gene editing in B. subtilis is reported, providing evidence that the nuclease‐mediated DNA double‐strand break acts as a counterselection mechanism after homologous recombination of the donor DNA.
Development and application of CRISPR/Cas genome editing system in the construction of prokaryotic microbial cell factories
TLDR
The establishment, optimization and applications of CRISPR-based genome editing tools in the construction of prokaryotic microbial cell factories are introduced and feasible solutions are proposed based on the discussion about the problems existed inCRISPR/Cas systems.
Temporal compartmentalization of viral infection in bacterial cells
TLDR
These findings show that bacteriophages restructure the crowded host cytoplasm to confine at different cellular locations the sequential processes that are essential for their multiplication.
...
1
2
...

References

SHOWING 1-10 OF 25 REFERENCES
The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
TLDR
The results show that active CRISPR/Cas systems can be transferred across distant genera and provide heterologous interference against invasive nucleic acids and can be leveraged to develop strains more robust against phage attack, and safer organisms less likely to uptake and disseminate plasmid-encoded undesirable genetic elements.
CRISPR: new horizons in phage resistance and strain identification.
TLDR
Hypervariable CRISPR loci provide insights into the phage and host population dynamics, and new avenues for enhanced phage resistance and genetic typing and tagging of industrial strains.
CRISPR-Cas systems: Prokaryotes upgrade to adaptive immunity.
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
TLDR
It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
TLDR
This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
TLDR
It is demonstrated that the Cas9–crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system introduces in vitro a double-strand break at a specific site in DNA containing a sequence complementary to crRNA, paving the way for engineering of universal programmable RNA-guided DNA endonucleases.
The roles of CRISPR-Cas systems in adaptive immunity and beyond.
Unravelling the structural and mechanistic basis of CRISPR–Cas systems
TLDR
This Review summarizes the recent structural and biochemical insights that have been gained for the three major types of CRISPR–Cas systems, which together provide a detailed molecular understanding of the unique and conserved mechanisms of RNA-guided adaptive immunity in bacteria and archaea.
Bacteriophage: genetics and molecular biology.
TLDR
This book focuses on the current rapid developments in bacteriophage research and provides a broad overview of the topics, including bioinformatics and genomics, phage in the environment, bacter iophage in medicine, transfer of phage DNA to the host, contribution to host phenotype and much more.
...
1
2
3
...