Design of FRET-based GFP probes for detection of protease inhibitors.

  title={Design of FRET-based GFP probes for detection of protease inhibitors.},
  author={Bin Zhang},
  journal={Biochemical and biophysical research communications},
  volume={323 2},
  • Bin Zhang
  • Published 2004 in
    Biochemical and biophysical research…
In this study, tandem Green fluorescent protein (GFP) fusion proteins were designed to detect proteolytic activity of thrombin based on the principle of fluorescence resonance energy transfer (FRET). The thrombin-specific recognition sequence, LVPR, was strategically placed in between a cyan-emitting mutant of the green fluorescent protein and an enhanced yellow-emitting fluorescent protein to allow thrombin-specific cleavage with detectable changes of FRET signal. A 4.6-fold increase of… CONTINUE READING