Temperature and humidity control of the intelligent greenhouse is an important link of monitoring. This paper uses Surplus 16-bit single chip microcomputer as the core control of the system. Combined with wireless transceiver chip NRF401, USB interface device PDIUSBD12 and temperature and humidity module YXHT300U, we designed a wireless temperature and humidity monitoring system which used in intelligent greenhouse. This system is composed of one central station and several base stations. The datum of temperature and humidity are collected, and then are transmitted to the central station from the base stations which are monitored by the central station via the wireless transceiver/receiver-nRF401. The datum is received from the base stations to central station, then are transmitted to the computer where they are analyzed via the USB interface. The status of the wireless transceiver is monitored by the system software which made its reliability improved.