Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel.

@article{Pini1998DesignAU,
  title={Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel.},
  author={Alessandro Pini and Francesca Viti and Annalisa Santucci and Barbara Carnemolla and Luciano Zardi and Paola Neri and Dario Neri},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 34},
  pages={
          21769-76
        }
}
We report the construction and the use of a phage display human antibody library (>3 x 10(8) clones) based on principles of protein design. A large repertoire of functional antibodies with similar properties was produced by appending short variable complementarity-determining region 3 (CDR3) onto the two antibody germ line segments most frequently found in human antibodies. With this strategy we concentrated sequence diversity in regions of the antibody structure that are centrally located in… CONTINUE READING
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