Design and semisynthesis of photoactivable split-GFP by incorporation of photocleavable functionality.


The design of proteins whose structure and function can be manipulated by the external stimuli has been of great interest in the field of protein engineering. In particular, caged proteins which can be activated by photo-irradiation become powerful tools for investigating a variety of biological events. Although protein caging is straightforward to render… (More)
DOI: 10.1002/bip.22304


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