Design and self-assembly of two-dimensional DNA crystals

  title={Design and self-assembly of two-dimensional DNA crystals},
  author={Erik Winfree and Furong Liu and Lisa A. Wenzler and Nadrian C. Seeman},
Molecular self-assembly presents a ‘bottom-up’ approach to the fabrication of objects specified with nanometre precision. DNA molecular structures and intermolecular interactions are particularly amenable to the design and synthesis of complex molecular objects. We report the design and observation of two-dimensional crystalline forms of DNA that self-assemble from synthetic DNA double-crossover molecules. Intermolecular interactions between the structural units are programmed by the design of… 

Self-assembly of two-dimensional DNA crystals

Self-assembly of synthetic oligonucleotides into two-dimensional lattices presents a ‘bottom-up’ approach to the fabrication of devices on nanometer scale. We report the design and observation of

DNA-based assembly lines and nanofactories.

  • F. Simmel
  • Chemistry, Biology
    Current opinion in biotechnology
  • 2012

3D DNA Crystals and Nanotechnology

Several DNA crystal structures are highlighted, the progress made in designing DNA crystals, and the current prospects and future directions of DNA crystals in nanotechnology are looked at.

Self-Assembly of Large-Scale Shape-Controlled DNA Nano-Structures

Abstract : We proposed to develop a methodology by using short synthetic DNA strands to build complex DNA nano-structures. In such a methodology, each short synthetic DNA strand, called DNA brick, is

Generation of protein lattices by fusing proteins with matching rotational symmetry.

It is shown that the genetic fusion of subunits from protein assemblies that have matching rotational symmetry generates species that can self-assemble into well-ordered, pre-determined one- and two-dimensional arrays that are stabilized by extensive intermolecular interactions.

DNA Crystals and Nanotechnology

Several DNA crystal structures are highlighted, the progress made in designing DNA crystals, and the current prospects and future directions of DNA crystals in nanotechnology are looked at.

Rapid Chiral Assembly of Rigid DNA Building Blocks for Molecular Nanofabrication

A family of DNA tetrahedra, less than 10 nanometers on a side, that can self-assemble in seconds with near-quantitative yield of one diastereomer are reported that can be connected by programmable DNA linkers.



Molecular self-assembly and nanochemistry: a chemical strategy for the synthesis of nanostructures.

The ability to prepare structures in the upper part of this range of sizes would open a route to structures comparable in size (and perhaps complementary in function) to those that can be prepared by microlithography and other techniques of microfabrication.

Organization of 'nanocrystal molecules' using DNA

A strategy for the synthesis of 'nanocrystal molecules', in which discrete numbers of gold nanocrystals are organized into spatially defined structures based on Watson-Crick base-pairing interactions is described.

Construction of a DNA-Truncated Octahedron

A covalently closed molecular complex whose double-helical edges have the connectivity of a truncated octahedron has been assembled from DNA on a solid support. This three-connected Archimedean solid

Synthesis from DNA of a molecule with the connectivity of a cube

This work reports the construction from DNA of a covalently closed cube-like molecular complex containing twelve equal-length double-helical edges arranged about eight vertices, the first construction of a closed polyhedral object from DNA.

The design of a biochip: a self-assembling molecular-scale memory device.

A design for a biochip memory device based on known materials and existing principles is presented, which relies on the self-assembly of the nucleic acid junction system, which acts as the scaffolding for a molecular wire consisting of polyacetylene-like units.

Oligonucleotide-directed self-assembly of proteins: semisynthetic DNA--streptavidin hybrid molecules as connectors for the generation of macroscopic arrays and the construction of supramolecular bioconjugates.

The use of nucleic acid sequences as a template for the formation of an array of proteins is further demonstrated on two size scales, and the generation of supramolecular bioconjugates was shown by quantitative measurements and gel-retardation assays.

A DNA-based method for rationally assembling nanoparticles into macroscopic materials

A method for assembling colloidal gold nanoparticles rationally and reversibly into macroscopic aggregates by using the specificity of DNA interactions to direct the interactions between particles of different size and composition is described.

Atomic force microscopy of oriented linear DNA molecules labeled with 5nm gold spheres

The gold spheres facilitate orientation of the DNA molecules on the mica surface to which they are absorbed and are potentially useful as internal height standards and as high resolution gene or sequence specific tags.

Nucleic acid junctions and lattices.

  • N. Seeman
  • Biology
    Journal of theoretical biology
  • 1982

Three-arm nucleic acid junctions are flexible.

It is shown here that junctions which contain three double helical arms can be enzymatically oligomerized, using conventional sticky-ended ligation procedures, to create larger complexes.