Design and expression of a QconCAT protein to validate Hi3 protein quantification of influenza vaccine antigens.

@article{Smith2016DesignAE,
  title={Design and expression of a QconCAT protein to validate Hi3 protein quantification of influenza vaccine antigens.},
  author={Daryl G S Smith and Genevi{\`e}ve Gingras and Yves Aubin and Terry D Cyr},
  journal={Journal of proteomics},
  year={2016},
  volume={146},
  pages={
          133-40
        }
}
UNLABELLED Quantification of the antigens hemagglutinin and neuraminidase in influenza vaccines has been reported using an antibody-free liquid chromatography-mass spectrometry (LC-MS) based method known as MS(E) "Hi3". This approach is based on the average signal intensity of the three most intense tryptic peptides relative to a primary standard. This strategy assumes that the Hi3 signal responses are consistent for all proteins, and therefore comparable to a spiked reference for absolute… CONTINUE READING