Design and creation of new nanomaterials for therapeutic RNAi.

Abstract

RNA interference is an evolutionarily conserved gene-silencing phenomenon that shows great promise for developing new therapies. However, the development of small interfering RNA (siRNA)-based therapies needs to overcome two barriers and be able to (i) identify chemically stable and effective siRNA sequences and (ii) efficiently silence target genes with siRNA doses that will be clinically feasible in humans. Here, we report the design and creation of interfering nanoparticles (iNOPs) as new systemic gene-silencing agents. iNOPs have two subunits: (i) a well-defined functionalized lipid nanoparticle as a delivery agent and (ii) a chemically modified siRNA for sustained silencing in vivo. When we injected iNOPs containing only 1-5 mg kg(-1) siRNA into mice, an endogenous gene for apolipoprotein B (apoB) was silenced in liver, plasma levels of apoB decreased, and total plasma cholesterol was lowered. iNOP treatment was nontoxic and did not induce an immune response. Our results show that these iNOPs can silence disease-related endogenous genes in clinically acceptable and therapeutically affordable doses.

050200920102011201220132014201520162017
Citations per Year

142 Citations

Semantic Scholar estimates that this publication has 142 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Baigude2007DesignAC, title={Design and creation of new nanomaterials for therapeutic RNAi.}, author={Huricha Baigude and Joshua Mccarroll and Chao-shun Yang and Pamela M Swain and Tariq M Rana}, journal={ACS chemical biology}, year={2007}, volume={2 4}, pages={237-41} }