Design and construction of highly stable, protease-resistant chimeric avidins.

  title={Design and construction of highly stable, protease-resistant chimeric avidins.},
  author={Vesa P. Hyt{\"o}nen and Juha A. E. M{\"a}{\"a}tt{\"a} and Thomas K. M. Nyholm and Oded Livnah and Yael Eisenberg-Domovich and David E. Hyre and Henri R. Nordlund and Jarno H{\"o}rh{\"a} and Einari A. Niskanen and Tiina A Paldanius and Tuomas Kulomaa and Eevaleena J Porkka and Patrick S. Stayton and Olli H. Laitinen and Markku S. Kulomaa},
  journal={The Journal of biological chemistry},
  volume={280 11},
The chicken avidin gene family consists of avidin and seven separate avidin-related genes (AVRs) 1-7. Avidin protein is a widely used biochemical tool, whereas the other family members have only recently been produced as recombinant proteins and characterized. In our previous study, AVR4 was found to be the most stable biotin binding protein thus far characterized (T(m) = 106.4 degrees C). In this study, we studied further the biotin-binding properties of AVR4. A decrease in the energy barrier… CONTINUE READING

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