Design and cloning of a synthetic gene for the flounder antifreeze protein and its expression in plant cells.

@article{Georges1990DesignAC,
  title={Design and cloning of a synthetic gene for the flounder antifreeze protein and its expression in plant cells.},
  author={Fawzy Georges and Muhammad Saleem and Adrian Cutler},
  journal={Gene},
  year={1990},
  volume={91 2},
  pages={159-65}
}
A synthetic gene coding for the winter flounder antifreeze protein (AFP) has been constructed. A new strategy for the synthesis has been employed such that one strand of the duplex was chemically synthesized and the other was produced enzymatically by chain extension. The chemically synthesized blocks were constructed so that the second strand was self… CONTINUE READING