Design and characterization of novel trypsin-resistant firefly luciferases by site-directed mutagenesis.

Abstract

Firefly luciferase (EC.1.13.12.7) from Photinus pyralis is a single polypeptide chain (62 kDa), responsible for emission of yellow-green (557 nm) light, known to be most efficient bioluminescence system that make it an excellent tool for reporter in nano-system biology. However, it is very sensitive to proteolytic degradation, which reduces its… (More)
DOI: 10.1093/protein/gzp047

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