Design and characterization of electrochromic membrane probes.

  • Leslie M. Loew
  • Published 1982 in Journal of biochemical and biophysical methods


Electrochronic membrane probes display a spectroscopic response to membrane potential by a direct electronic mechanism. This allows such probes to be designed a priori via quantum-chemical techniques. The detailed behavior of potentiometric optical probes can be elucidated with an apparatus based on phase-sensitive detection from a hemispherical lipid bilayer; several different types of response spectra can be obtained with this apparatus allowing distinction between the electrochromic mechanism and the more common molecular-motion based mechanisms. The development of 'fast' potentiometric dyes has now reached a stage where practical and exciting applications are rapidly appearing. It is anticipated that the emergence of a complementary set of electrochromic probes will lead to new applications; in particular, it may be possible to elucidate the molecular events which underlie biological or physiological phenomena.


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@article{Loew1982DesignAC, title={Design and characterization of electrochromic membrane probes.}, author={Leslie M. Loew}, journal={Journal of biochemical and biophysical methods}, year={1982}, volume={6 3}, pages={243-60} }