Design and characterization of a fluorescent ghrelin analog for imaging the growth hormone secretagogue receptor 1a

@article{Mcgirr2011DesignAC,
  title={Design and characterization of a fluorescent ghrelin analog for imaging the growth hormone secretagogue receptor 1a},
  author={Rebecca Mcgirr and Mark S. McFarland and J. McTavish and L. Luyt and S. Dhanvantari},
  journal={Regulatory Peptides},
  year={2011},
  volume={172},
  pages={69-76}
}
  • Rebecca Mcgirr, Mark S. McFarland, +2 authors S. Dhanvantari
  • Published 2011
  • Biology, Medicine
  • Regulatory Peptides
  • Ghrelin is a 28-amino acid peptide hormone produced in the stomach. It binds to the growth hormone secretagogue receptor 1a (GHS-R1a), a class A G-protein-coupled receptor. In the present study, we describe the design, synthesis and characterization of a truncated, 18-amino acid analog of ghrelin conjugated to a fluorescent molecule, fluorocein isothiocyanate (FITC), through the addition of a lysine at its C terminus ([Dpr(octanoyl)(3), Lys(fluorescein)(19)]ghrelin(1-19)). Receptor binding… CONTINUE READING
    39 Citations
    Novel and Conventional Receptors for Ghrelin, Desacyl-Ghrelin, and Pharmacologically Related Compounds
    • 71
    • PDF
    Ghrelin binding to serum albumin and its biological impact
    • 5

    References

    SHOWING 1-10 OF 39 REFERENCES
    Ala-scan of ghrelin (1–14): interaction with the recombinant human ghrelin receptor
    • 34
    Fluorine and rhenium substituted ghrelin analogues as potential imaging probes for the growth hormone secretagogue receptor.
    • 27
    Design, synthesis and in vitro characterization of Glucagon-Like Peptide-1 derivatives for pancreatic beta cell imaging by SPECT.
    • 17
    Structural Divergence of Human Ghrelin
    • 290
    • PDF
    Ghrelin is a growth-hormone-releasing acylated peptide from stomach
    • 7,394
    Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a.
    • 61
    The truncated ghrelin receptor polypeptide (GHS-R1b) acts as a dominant-negative mutant of the ghrelin receptor.
    • 139
    Heterogeneity of Ghrelin/Growth Hormone Secretagogue Receptors
    • 104