• DOI:10.1038/s41598-017-15711-z
Corpus ID: 7250583

Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity

@article{Hansen2017DesignAA,
  title={Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity},
  author={Simon Hansen and Jakob C St{\"u}ber and P. Ernst and Alexander Koch and Daniel Bojar and A. Batyuk and A. Pl{\"u}ckthun},
  journal={Scientific Reports},
  year={2017},
  volume={7}
}

Simon Hansen, Jakob C Stüber, +4 authors A. Plückthun

Published 2017

Chemistry, Medicine

Scientific Reports

Green fluorescent protein (GFP) fusions are pervasively used to study structures and processes. Specific GFP-binders are thus of great utility for detection, immobilization or manipulation of GFP-fused molecules. We determined structures of two designed ankyrin repeat proteins (DARPins), complexed with GFP, which revealed different but overlapping epitopes. Here we show a structure-guided design strategy that, by truncation and computational reengineering, led to a stable construct where both… CONTINUE READING

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