Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity

@article{Hansen2017DesignAA,
  title={Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity},
  author={Simon Hansen and Jakob C St{\"u}ber and P. Ernst and Alexander Koch and Daniel Bojar and A. Batyuk and A. Pl{\"u}ckthun},
  journal={Scientific Reports},
  year={2017},
  volume={7}
}
  • Simon Hansen, Jakob C Stüber, +4 authors A. Plückthun
  • Published 2017
  • Chemistry, Medicine
  • Scientific Reports
  • Green fluorescent protein (GFP) fusions are pervasively used to study structures and processes. Specific GFP-binders are thus of great utility for detection, immobilization or manipulation of GFP-fused molecules. We determined structures of two designed ankyrin repeat proteins (DARPins), complexed with GFP, which revealed different but overlapping epitopes. Here we show a structure-guided design strategy that, by truncation and computational reengineering, led to a stable construct where both… CONTINUE READING
    24 Citations

    Figures, Tables, and Topics from this paper

    Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria.
    • 4
    • PDF
    DARPins recognize mTFP1 as novel reagents for in vitro and in vivo protein manipulations
    • 4
    • PDF
    DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations
    • 1
    • PDF
    Generation of ordered protein assemblies using rigid three-body fusion
    • 2
    • PDF

    References

    SHOWING 1-10 OF 76 REFERENCES
    Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools.
    • 19
    • PDF
    FRET-based in vivo screening for protein folding and increased protein stability.
    • 47
    Modulation of protein properties in living cells using nanobodies
    • 363
    • PDF
    The molecular structure of green fluorescent protein
    • 1,065