Design and application of a synthetic DNA standard for real-time PCR analysis of microbial communities in a biogas digester

@article{May2015DesignAA,
  title={Design and application of a synthetic DNA standard for real-time PCR analysis of microbial communities in a biogas digester},
  author={Tobias May and Mareike Koch-Singenstreu and Johannes Ebling and Robbin Stantscheff and Liane M{\"u}ller and Frieder Jacobi and Daniela Polag and Frank Keppler and Helmut K{\"o}nig},
  journal={Applied Microbiology and Biotechnology},
  year={2015},
  volume={99},
  pages={6855-6863}
}
A synthetic DNA fragment containing primer binding sites for the quantification of ten different microbial groups was constructed and evaluated as a reliable enumeration standard for quantitative real-time PCR (qPCR) analyses. This approach has been exemplary verified for the quantification of several methanogenic orders and families in a series of samples drawn from a mesophilic biogas plant. Furthermore, the total amounts of bacteria as well as the number of sulfate-reducing and propionic… CONTINUE READING
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