Design and Application of a Novel High-throughput Screening Technique for 1-Deoxynojirimycin

@inproceedings{Jiang2015DesignAA,
  title={Design and Application of a Novel High-throughput Screening Technique for 1-Deoxynojirimycin},
  author={Peixia Jiang and Shanshan Mu and Heng Li and Youhai Li and Congmin Feng and Jian-Ming Jin and Shuang-Yan Tang},
  booktitle={Scientific reports},
  year={2015}
}
High-throughput screening techniques for small molecules can find intensive applications in the studies of biosynthesis of these molecules. A sensitive, rapid and cost-effective technique that allows high-throughput screening of endogenous production of the natural iminosugar 1-deoxynojirimycin (1-DNJ), an α-glucosidase inhibitor relevant to the pharmaceutical industry, was developed in this study, based on the inhibitory effects of 1-DNJ on the activity of the β-glycosidase LacS from… CONTINUE READING