Design, production, and characterization of an engineered biotin ligase (BirA) and its application for affinity purification of staphylokinase produced from Bacillus subtilis via secretion.

@article{Wu2002DesignPA,
  title={Design, production, and characterization of an engineered biotin ligase (BirA) and its application for affinity purification of staphylokinase produced from Bacillus subtilis via secretion.},
  author={S C Wu and Jonathan C Yeung and Peter M. Hwang and S Wong},
  journal={Protein expression and purification},
  year={2002},
  volume={24 3},
  pages={357-65}
}
A major attraction in using Bacillus subtilis as an expression host for heterologous protein production is its ability to secrete extracellular proteins into the culture medium. To take full advantage of this system, an efficient method for recovering the target protein is crucial. For secretory proteins which cannot be purified by a simple scheme, in vitro… CONTINUE READING