Design, construction and extracellular expression of L-asparaginase from Dickeya chrysanthemi in yeast

@inproceedings{Farias2018DesignCA,
  title={Design, construction and extracellular expression of L-asparaginase from Dickeya chrysanthemi in yeast},
  author={Brian Effer Jorge Farias and Gisele Monteiro},
  year={2018}
}
L (L-ASNase) is an important enzyme used as a biopharmaceutical to treat acute lymphoblastic leukemia (ALL). Currently there are two L-ASNase approved by FDA: native and of bacterial origin, both from E. coli and D. chrysanthemi. Due to L-ASNase’s immunogenic effects, it is necessary to seek alternatives such as recombinant expression in yeast. This expression system can provide extracelullar secretion and glycosilation process, which can decrease immunogenicity and facilitate downstream… CONTINUE READING

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