Design, construction and characterization of a set of insulated bacterial promoters

@inproceedings{Davis2011DesignCA,
  title={Design, construction and characterization of a set of insulated bacterial promoters},
  author={Joseph H Davis and Adam J. Rubin and Robert T. Sauer},
  booktitle={Nucleic acids research},
  year={2011}
}
We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design insulates these promoters from the stimulatory or repressive effects of many 5'- or 3'-sequence elements. We show that different promoters from our library produce constant relative levels of two… CONTINUE READING

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