Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

@inproceedings{LossMorais2012DescriptionOP,
  title={Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets},
  author={Guilherme Loss-Morais and Peter M. Waterhouse and Rogerio Margis},
  booktitle={Biology Direct},
  year={2012}
}
tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on… CONTINUE READING
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