Derivatization of compounds at the origin of thin-layer plates with fluorescamine.

A method has been developed for the detection of compounds with primary amino groups on thin-layer chromatography (TLC) plates with fluorescamine. The compounds dissolved in buffer were applied to TLC plates and derivatized at the origin by developing with or dipping into an acetone-hexane solution of fluorescamine. Virtually all fluorescamine derivatives… CONTINUE READING