Derivation of pluripotent stem cells from cultured human primordial germ cells.

@article{Shamblott1998DerivationOP,
  title={Derivation of pluripotent stem cells from cultured human primordial germ cells.},
  author={Michael J. Shamblott and Joyce Axelman and S Wang and Elizabeth M Bugg and John W. Littlefield and Peter J Donovan and Paul D. Blumenthal and George R. Huggins and John D. Gearhart},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 23},
  pages={13726-31}
}
Human pluripotent stem cells would be invaluable for in vitro studies of aspects of human embryogenesis. With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. Initially, single PGCs in culture were visualized by… CONTINUE READING
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