Derivation of pluripotent stem cells from cultured human primordial germ cells.

  title={Derivation of pluripotent stem cells from cultured human primordial germ cells.},
  author={Michael J. Shamblott and Joyce Axelman and S Wang and Elizabeth M Bugg and John W. Littlefield and Peter J Donovan and Paul D. Blumenthal and George R. Huggins and John D. Gearhart},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={95 23},
Human pluripotent stem cells would be invaluable for in vitro studies of aspects of human embryogenesis. With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. Initially, single PGCs in culture were visualized by… CONTINUE READING
Highly Influential
This paper has highly influenced 27 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS


Publications citing this paper.
Showing 1-10 of 450 extracted citations

Transfection of Human Embryonic Germ(EG) Cells with Human Cardiac alpha-Actin Promoter Driving pEGFP-N1

2009 2nd International Conference on Biomedical Engineering and Informatics • 2009
View 4 Excerpts
Highly Influenced

Human embryonic germ cells isolation from early stages of post-implantation embryos

Cell and Tissue Research • 2004
View 7 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…