Derivation of genetically modified human pluripotent stem cells with integrated transgenes at unique mapped genomic sites

Abstract

Many applications in human pluripotent stem cell (PSC) research require the genetic modification of PSCs to express a transgene in a stable and dependable manner. Random transgene integration commonly results in unpredictable and heterogeneous expression. We describe a protocol for the derivation of clonal populations of human embryonic stem cells or… (More)
DOI: 10.1038/nprot.2011.362

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