Derivation of a physical map of the chromosome of Bordetella pertussis Tohama I.

Abstract

We have used pulsed-field gel electrophoresis to derive a restriction map of the chromosome of Bordetella pertussis for the enzymes XbaI, SpeI, PacI, and PmeI, which cleave 25, 16, 2, and 1 times, respectively. The apparent size of the genome is 3,750 kb. The positions of genes for major virulence determinants in the vir regulon and of some housekeeping genes were determined. Apart from the previously known linkage of the vir and fha loci, no significant linkage of virulence genes was demonstrated.

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Cite this paper

@article{Stibitz1992DerivationOA, title={Derivation of a physical map of the chromosome of Bordetella pertussis Tohama I.}, author={Scott E Stibitz and T L Garletts}, journal={Journal of bacteriology}, year={1992}, volume={174 23}, pages={7770-7} }