Derivation of Human Embryonic Stem Cells from Developing and Arrested Embryos

@article{Zhang2006DerivationOH,
  title={Derivation of Human Embryonic Stem Cells from Developing and Arrested Embryos},
  author={X. Zhang and P. Stojkovic and S. Przyborski and Michael Cooke and L. Armstrong and M. Lako and M. Stojkovic},
  journal={STEM CELLS},
  year={2006},
  volume={24}
}
Human embryonic stem cells (hESC) hold huge promise in modern regenerative medicine, drug discovery, and as a model for studying early human development. However, usage of embryos and derivation of hESC for research and potential medical application has resulted in polarized ethical debates since the process involves destruction of viable developing human embryos. Here we describe that not only developing embryos (morulae and blastocysts) of both good and poor quality but also arrested embryos… Expand
Derivation of human embryonic stem cell lines from poor quality embryos.
TLDR
A simple protocol is described for isolating pluripotent stem cells from abnormally developed grade III human embryos that are an unutilized byproduct of in vitro fertility treatment and derived two sibling hESC lines that represent the Indian ethnic background and show stable phenotype upon long-term continuous culture of over 225 passages. Expand
Comparison of three embryo culture methods for derivation of human embryonic stem cells from discarded embryos.
TLDR
It is demonstrated that culturing discarded embryos as multiple embryo aggregates was more profitable for outgrowth and derivation of ESC line than culturing these as single embryo or blastomeres. Expand
Derivation of Euploid Human Embryonic Stem Cells from Aneuploid Embryos
TLDR
This study used blastocyst‐stage embryos diagnosed as aneuploid in preimplantation genetic screening (PGS) to isolate new lines of HESCs, and karyotype analysis of the HESC lines that were derived showed that the cell lines carry a normal euploid karyotypes. Expand
Non-viable human embryos as a source of viable cells for embryonic stem cell derivation.
TLDR
It is shown that arrested embryos do not resume normal development during extended culture, yet most of them contain a substantial number of living cells on embryonic day 6, suggesting that this class of non-viable embryos could be a rich source of viable cells for derivation of hESC lines. Expand
Pluripotent Stem Cell Banks
TLDR
The emerging demands of stem cell research and applications require the establishment and cooperation of centralized banks at a translational and even global scale to ensure the availability of high-quality hPSC using standardized state-of-the-art methods and strategies to deal with a heterogeneous regulatory, ethical and legal landscape. Expand
Derivation of the first Swiss human embryonic stem cell line from a single blastomere of an arrested four-cell stage embryo.
TLDR
The first Swiss hESC line, called CH-ES1, derived from a single blastomere of an arrested four-cell-stage embryo is reported, which expresses ESC markers of pluripotency and differentiated into all three germ layers in embryoid bodies in vitro and in teratomas in vivo. Expand
Derivation of new human embryonic stem cell lines from preimplantation genetic screening and diagnosis-analyzed embryos
TLDR
Results indicate that hESC lines can be derived from PGD/PGS-analyzed embryos that are destined to be discarded and can serve as an alternative source for normal euploid lines. Expand
FOR DERIVATION OF HUMAN EMBRYONIC STEM CELLS
Human embryonic stem (hES) cells are self-renewing cells that can give rise to many types of cells found in the body. These cells can only be extracted from pre-implantation embryos. The timing ofExpand
Derivation of human embryonic stem cell lines from single blastomeres of low-quality embryos by direct plating
TLDR
Plating of blastomeres from LQ embryos directly into the hESC-CS is a feasible method for deriving h ESC lines and shows a pluripotent phenotype, including the ability to differentiate into all three germ layers in vitro and in vivo. Expand
Alternative Sources of Human Embryonic Stem Cells
TLDR
A retrospective analysis of the morphological progression from ED5 to ED6 in 2,480 embryos that are rejected for clinical use shows that nonviable embryos defined as poor do not improve with extended in vitro culture and yet retain the capacity to yield human ES cell lines despite arrested development. Expand
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TLDR
It is reported here that the novel three‐step culture conditions successfully support the development of day‐8 human blastocysts, which possess significantly (p <.01) more ICM cells than day‐6 Blastocysts. Expand
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TLDR
These hESC lines are an important resource for functional genomics, drug screening, and, perhaps eventually, cell and gene therapy, and the prospects for use in regenerative medicine are significant. Expand
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TLDR
Four human pluripotent embryonic stem cells are derived from poor‐quality embryos that in the course of routine clinical practice would have been discarded, and maintained their developmental potential to form trophoblast and somatic cells, including cardiac muscle and neuronal tissue. Expand
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TLDR
It is suggested that a subset with poor quality day 3 embryos judged on the basis of morphological assessment can form blastocysts and give rise to hES cell lines. Expand
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TLDR
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TLDR
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TLDR
A new stem-cell line was derived from human embryos under completely cell and serum free conditions, eliminating exposure of human embryonic stem cells and their progeny to animal and human feeder layers, and thus the risk of contamination with pathogenic agents capable of transmitting diseases to patients. Expand
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Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primateExpand
Downregulation of NANOG Induces Differentiation of Human Embryonic Stem Cells to Extraembryonic Lineages
TLDR
The findings suggest that NANOG acts as a gatekeeper of pluripotency in human embryonic stem and carcinoma cells by preventing their differentiation to extraembryonic endoderm and trophectoderm lineages. Expand
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TLDR
This method, which requires neither immuno nor mechanical removal of the trophectoderm, may facilitate the derivation of hESC lines in general, and those from abnormal embryos in particular. Expand
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