Derivation and morphological characterization of mouse spermatogonial stem cell lines.

  title={Derivation and morphological characterization of mouse spermatogonial stem cell lines.},
  author={Takehiko Ogawa and Masako Ohmura and Yoichi Tamura and Kaoru Kita and Kazuyuki Ohbo and Toshio Suda and Yoshinobu Kubota},
  journal={Archives of histology and cytology},
  volume={67 4},
Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the… CONTINUE READING
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