Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark

@article{Harkness2010DerivationAC,
  title={Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark},
  author={Linda Harkness and Iben Anne Rasmussen and Karin Erb and Moustapha Kassem},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={2010},
  volume={46},
  pages={259-268}
}
The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to… CONTINUE READING