Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions

  title={Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions},
  author={Caroline Kubaczka and Claire E. Senner and Marcos Jes{\'u}s Ara{\'u}zo-Bravo and Neha Sharma and Peter Kuckenberg and Astrid Becker and Andreas Zimmer and Oliver Bruestle and Michael Peitz and Myriam Hemberger and Hubert Schorle},
  booktitle={Stem cell reports},
Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and… CONTINUE READING
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