Derivation, maintenance, and characterization of rat embryonic stem cells in vitro.

@article{Schulze2006DerivationMA,
  title={Derivation, maintenance, and characterization of rat embryonic stem cells in vitro.},
  author={Maren Schulze and Hendrik Ungefroren and Michael Bader and Fred Faendrich},
  journal={Methods in molecular biology},
  year={2006},
  volume={329},
  pages={45-58}
}
The in vitro differentiation of mouse embryonic stem (ES) cells into different somatic cell types such as neurons, endothelial cells, or myocytes is well established, and many mouse ES cell lines have been created so far. The establishment of rat ES cell lines, however, has proven to be difficult. Most attempts to culture rat ES cell lines and maintain them in an undifferentiated state have failed, so researchers were forced to abandon this system and use mouse ES cells. This chapter describes… CONTINUE READING

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