Deranged B12 metabolism: Studies of fibroblasts grown in tissue culture

@article{Mudd1970DerangedBM,
  title={Deranged B12 metabolism: Studies of fibroblasts grown in tissue culture},
  author={S. Mudd and B. Uhlendorf and K. Hinds and H. Levy},
  journal={Biochemical Medicine},
  year={1970},
  volume={4},
  pages={215-239}
}
Abstract Studies are reported of fibroblasts cultured from the skin of a patient with abnormalities of sulfur amino acid and of methylmalonic acid metabolism. These cells are unable to grow as do normal cells if methionine in the medium is replaced by homocystine. Addition of hydroxy-B12, 1 μg/ml, to the medium allowed the cells of the patient to grow slowly. Extracts of the cells contained a normal concentration of cystathionine synthase, but an abnormally low concentration of holoenzyme for… Expand
Cobalamins in Fibroblasts Cultured from Normal Control Subjects and Patients with Methylmalonic Aciduria
Extract: The intracellular content and proportional distribution of B12 (cobalamin) derivatives in fibroblasts cultured from patients with various forms of methylmalonic aciduria, as well as fromExpand
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Abstract Several enzymes have been studied in the tissues of a patient who had a combination of increased homocystine and cystathionine and decreased methionine in plasma, tissues, and urine. ThisExpand
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