Depth resolution of the fluorescent confocal scanning optical microscope.

@article{Kimura1990DepthRO,
  title={Depth resolution of the fluorescent confocal scanning optical microscope.},
  author={Shigeya Kimura and Chusuke Munakata},
  journal={Applied optics},
  year={1990},
  volume={29 4},
  pages={
          489-94
        }
}
The fluorescent confocal scanning optical microscope is capable of 3-D observation, for which depth discrimination is vital. To characterize the depth discrimination capability of this microscope, intensity variations depending on fluorescence wavelength are simulated for fluorescent films being moved in the depth direction. Also, the resolution between two films is calculated for a range of fluorescence wavelengths. Results of experiments using photoresist films are compared to simulations. As… CONTINUE READING
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