Depletion of naive T cells using clinical grade magnetic CD45RA beads: a new approach for GVHD prophylaxis

@article{Teschner2014DepletionON,
  title={Depletion of naive T cells using clinical grade magnetic CD45RA beads: a new approach for GVHD prophylaxis},
  author={Daniel Teschner and Eva Distler and Daniela Wehler and Michaela Frey and D Marandiuc and Kirsten Langeveld and Matthias Theobald and S{\"o}ren Thomas and Wolfgang Herr},
  journal={Bone Marrow Transplantation},
  year={2014},
  volume={49},
  pages={138-144}
}
Depletion of naive T cells from donor leukapheresis products (LPs) aims at the reduction of alloreactivity, while preserving memory T-cell reactivity (for example, to pathogens). This study established the immunomagnetic depletion procedure under clean room conditions using CD45RA beads and analyzed LPs of six donors for cell composition and functional immune responses. CD45RA depletion resulted in 3.4–4.7 log (median 4.4) reduction of CD45RA+ T cells, thereby eliminating naive and late… CONTINUE READING