Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis and recruitment of both clathrin and clathrin adaptors.

@article{Lee2005DepletionOG,
  title={Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis and recruitment of both clathrin and clathrin adaptors.},
  author={Dong-won Lee and Xiaohong Zhao and Fang Zhang and Evan Eisenberg and Lois E. Greene},
  journal={Journal of cell science},
  year={2005},
  volume={118 Pt 18},
  pages={4311-21}
}
Cyclin G-associated kinase (GAK/auxilin 2), the ubiquitous form of the neuronal-specific protein auxilin 1, is an essential cofactor for the Hsc70-dependent uncoating of clathrin-coated vesicles. We have now investigated the effect of knocking down GAK in HeLa cells by vector-based small hairpin RNA. Functionally, depletion of GAK caused a marked decrease in internalization of both transferrin and epidermal growth factor and altered mannose 6-phosphate receptor trafficking, but had little… CONTINUE READING
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