Depletion and repletion of cortical tissue and dialysate 5-HT after reserpine.

Abstract

Reserpine (5 mg/kg s.c.) was given to rats kept under a reversed light-dark cycle and 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) determined in frontal cortex tissue and dialysate at various times after drug treatment. The decline and return of spontaneous locomotor activity was also measured. Tissue 5-HT was depleted to 16% of control values 24 hr after drug administration and had recovered to 61% of control after 21 days. Locomotion was profoundly reduced by 7 hr after reserpine but had returned to normal at 4 days. Dialysate 5-HT, both basal and its rise on potassium (K+) stimulation, was reduced at 1, 7 and 21 days after reserpine but the K+ stimulated increases (as % of control) did not rise above % tissue repletion, thus providing evidence against increased mobilization of the transmitter from the partially repleted vesicular stores. However, at 1 day after reserpine, basal dialysate 5-HT was proportionately less reduced than tissue 5-HT suggesting that release from a reserpine insensitive (extravesicular) pool was more effective than from the vesicular pool. At this time, the K(+)-stimulated rise of dialysate 5-HT was proportionately more reduced than tissue 5-HT. By 21 days, values converged so that % changes of the 3 compartments were the same suggesting that at this time both basal and K+ stimulated dialysate 5-HT was essentially all derived from the vesicular pool.

Statistics

0200400'98'00'02'04'06'08'10'12'14'16
Citations per Year

423 Citations

Semantic Scholar estimates that this publication has 423 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Heslop1994DepletionAR, title={Depletion and repletion of cortical tissue and dialysate 5-HT after reserpine.}, author={K E Heslop and Gerald Curzon}, journal={Neuropharmacology}, year={1994}, volume={33 3-4}, pages={567-73} }