Depleting dietary valine permits nonmyeloablative mouse hematopoietic stem cell transplantation

@article{Taya2016DepletingDV,
  title={Depleting dietary valine permits nonmyeloablative mouse hematopoietic stem cell transplantation},
  author={Yuki Taya and Yasunori Ota and Adam C. Wilkinson and Ayano Kanazawa and Hiroshi Watarai and Masataka Kasai and Hiromitsu Nakauchi and Satoshi Yamazaki},
  journal={Science},
  year={2016},
  volume={354},
  pages={1152 - 1155}
}
Hematopoietic stem cell maintenance requires the amino acid valine. How to maintain hematopoietic stem cells Hematopoiesis provides the body with a continuous supply of blood cells (see the Perspective by Sommerkamp and Trumpp). Taya et al. report that amino acid content is important for hematopoietic stem cell (HSC) maintenance in vitro and in vivo. Dietary valine restriction seems to “empty” the mouse bone marrow niche. Ito et al. used single-cell approaches and cell transplantation to… Expand
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References

SHOWING 1-10 OF 18 REFERENCES
Efficient Transplantation via Antibody-Based Clearance of Hematopoietic Stem Cell Niches
TLDR
Evidence is provided that, aside from immune barriers, donor HSC engraftment is restricted by occupancy of appropriate niches by host HSCs, and that extrapolation of these methods to humans may enable mild but effective conditioning regimens for transplantation. Expand
Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene therapy
TLDR
A novel human hematopoietic cell, the SCID–repopulating cell (SRC), a cell more primitive than most LTC–ICs and CFCs, that is capable of multilineage repopulation of the bone marrow of nonobese diabetic mice with severe combined immunodeficiency disease (NOD/SCID mice). Expand
Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice.
TLDR
Detailed flow cytometric analysis of the marrow of highly engrafted mice demonstrated both lymphoid and myeloid differentiation, as well as the retention of a significant fraction of CD34(+) CD38(-) cells, which should be useful for identification of the cellular and molecular mechanisms that regulate primitive human hematopoietic cells. Expand
Adult mouse hematopoietic stem cells: purification and single-cell assays
TLDR
Current protocols for the purification of CD34−/lowc-Kit+Sca-1+lineage marker− (CD34−KSL) cells, the HSC population making up approximately 0.005% of bone marrow cells in adult C557BL/6 mice, are described and immunostaining procedures for small numbers of HSCs are introduced, which are useful for signal transduction analysis. Expand
Conditioning regimens for hematopoietic cell transplantation: one size does not fit all.
TLDR
The definition of high-dose, reduced-intensity, and nonmyeloablative conditioning regimens, the most commonly used agents and combinations, and the evolution of some early regimens are provided. Expand
The bone marrow niche for haematopoietic stem cells
TLDR
The haematopoietic stem cell niche remains incompletely defined and beset by competing models, and outstanding questions concern the cellular complexity of the niche, the role of the endosteum and functional heterogeneity among perivascular microenvironments. Expand
SLAM Family Receptors Distinguish Hematopoietic Stem and Progenitor Cells and Reveal Endothelial Niches for Stem Cells
TLDR
This work compared the gene expression profiles of highly purified HSCs and non-self-renewing multipotent hematopoietic progenitors and found that both groups occupied multiple niches, including sinusoidal endothelium in diverse tissues. Expand
A clonogenic common myeloid progenitor that gives rise to all myeloid lineages
TLDR
The prospective identification, purification and characterization, using cell-surface markers and flow cytometry, of a complementary clonogenic common myeloid progenitor that gives rise to all myeloids lineages is reported. Expand
Granulocytopenia and Anemia in Rats Fed Diets of Low Casein Content
TLDR
In the correction of granulocytopenia in rats fed protein-free diets, L. casei factor alone was only slightly effective, and diets of higher casein content were ineffective under the experimental conditions described, but L. casesi factor combined with an 18-per cent casein-containing diet or a mixture of purified amino acids were found to be highly effective. Expand
L-serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons.
TLDR
It is demonstrated that l-serine (l-Ser) and glycine (Gly) also are glia-derived trophic factors that mediate the troPHic actions of glial cells on Purkinje neurons. Expand
...
1
2
...