Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4.

@article{Peled1999DependenceOH,
  title={Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4.},
  author={Amnon Peled and Isabelle Petit and Orit Kollet and Michal Magid and Tatyana Ponomaryov and Tamara V. Byk and Arnon Nagler and Herzl Ben‐Hur and Ariel Many and Leonard Shultz and Ofer Lider and Ronen Alon and Dov Zipori and Tsvee Lapidot},
  journal={Science},
  year={1999},
  volume={283 5403},
  pages={
          845-8
        }
}
Stem cell homing and repopulation are not well understood. The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 were found to be critical for murine bone marrow engraftment by human severe combined immunodeficient (SCID) repopulating stem cells. Treatment of human cells with antibodies to CXCR4 prevented engraftment. In vitro CXCR4-dependent migration to SDF-1 of CD34+CD38-/low cells correlated with in vivo engraftment and stem cell function. Stem cell factor and… 
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1,749 Citations

CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice.

The findings demonstrated the importance of the SDF-1/CX CR4 axis in the regulation of in vivo motility and development of human AML stem cells and identified CXCR4 neutralization as a potential treatment for AML.

Overexpression of CXCR4 on human CD34+ progenitors increases their proliferation, migration, and NOD/SCID repopulation.

The results suggest CXCR4 overexpression for improved definitive human stem cell motility, retention, and multilineage repopulation, which could be beneficial for in vivo navigation and expansion of hematopoietic progenitors.

CXCR 4 Regulates Migration and Development of Human Acute Myelogenous Leukemia Stem Cells in Transplanted NOD / SCID Mice

The findings demonstrated the importance of the SDF-1/CX CR4 axis in the regulation of in vivo motility and development of human AML stem cells and identified CXCR4 neutralization as a potential treatment for AML.

Mechanism of Human Stem Cell Migration and Repopulation of NOD/SCID and B2mnull NOD/SCID Mice

  • T. Lapidot
  • Biology
    Annals of the New York Academy of Sciences
  • 2001
A major role is suggested for SDF‐1/CXCR4 interactions in murine stem cell homing from the fetal liver into the bone marrow and its repopulation during development and in vivo homing and repopulating in transplanted immune‐deficient mice.

The essential roles of the chemokine SDF-1 and its receptor CXCR4 in human stem cell homing and repopulation of transplanted immune-deficient NOD/SCID and NOD/SCID/B2mnull mice

The essential roles of the chemokine SDF-1 and its receptor CXCR4 which mediate and regulate stem cell homing and repopulation will be discussed.

Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor.

The present study may provide a novel culture method for the expansion of human transplantable hematopoietic stem cells suitable for clinical applications.

Chemokine-mobilized adult stem cells; defining a better hematopoietic graft

In mice, the HSC and progenitor cells (HPC) mobilized by the CXCR2 receptor agonist GROβ can be harvested within minutes of administration and show significantly lower levels of apoptosis, enhanced homing to marrow, expression of more activated integrin receptors and superior repopulation kinetics and more competitive engraftment than the equivalent cells mobilized by G-CSF.

Treatment of circulating CD34(+) cells with SDF-1alpha or anti-CXCR4 antibody enhances migration and NOD/SCID repopulating potential.

Mobilization of hematopoietic stem and progenitor cells using inhibitors of CXCR4 and VLA-4

The development of small-molecule CXCR4 and VLA-4 inhibitors are discussed and how they may improve the utility and convenience of peripheral blood stem cell transplantation.

Short-term culture of umbilical cord blood-derived CD34 cells enhances engraftment into NOD/SCID mice through increased CXCR4 expression.

It is suggested that increased cell surface expression of CXCR4 on CD34(+) cells improved the engraftment of human umbilical CB cells into bone marrow through enhanced homing activity.
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