Dependence of complex formation of (1-->3)-beta-D-glucan with congo red on temperature in alkaline solutions.

@article{Ogawa1994DependenceOC,
  title={Dependence of complex formation of (1-->3)-beta-D-glucan with congo red on temperature in alkaline solutions.},
  author={Kozo Ogawa and Takaaki Dohmaru and Toshifumi Yui},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1994},
  volume={58 10},
  pages={
          1870-2
        }
}
The formation of a complex between (1-->3)-beta-D-glucan and congo red in dilute alkaline solutions (0.1 and 0.15 M sodium hydroxide) where the glucan takes an ordered conformation was studied by measuring visible absorption spectra of the solutions at various temperatures between 15 and 35 degrees C. The average number of the glucose residues forming the binding site to accommodate one dye molecule decreased with increasing temperature or decreasing alkaline concentration, suggesting possible… CONTINUE READING
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