Dependence of 3-D optical transfer functions on the pinhole radius in a fluorescent confocal optical microscope.

@article{Kimura1990DependenceO3,
  title={Dependence of 3-D optical transfer functions on the pinhole radius in a fluorescent confocal optical microscope.},
  author={Shigeya Kimura and C Munakata},
  journal={Applied optics},
  year={1990},
  volume={29 20},
  pages={3007-11}
}
The resolution of a fluorescent confocal scanning optical microscope (CSOM) is superior to that of a conventional fluorescent optical microscope. To attain this superiority, the fluorescent CSOM uses a pinhole in front of the detector. Thus, the resolution of the CSOM is dependent on the pinhole radius. Three-dimensional optical transfer functions are calculated for the various radii to elucidate this dependence. The results show that a CSOM with a radius smaller than ~1 optical unit has a… CONTINUE READING