Deoxyuridine is generated preferentially in the nontranscribed strand of DNA from cells expressing activation-induced cytidine deaminase.

@article{Martomo2005DeoxyuridineIG,
  title={Deoxyuridine is generated preferentially in the nontranscribed strand of DNA from cells expressing activation-induced cytidine deaminase.},
  author={Stella A. Martomo and Dongtao Fu and William Yang and Nikhil S Joshi and Patricia J Gearhart},
  journal={Journal of immunology},
  year={2005},
  volume={174 12},
  pages={7787-91}
}
Activation-induced cytidine deaminase (AID) is required for somatic hypermutation and class switch recombination of Ig genes in B cells. Although AID has been shown to deaminate deoxycytidine to deoxyuridine in DNA in vitro, there is no physical evidence for increased uracils in DNA from cells expressing AID in vivo. We used several techniques to detect uracil bases in a gene that was actively transcribed in Escherichia coli cells expressing AID. Plasmid DNA containing the gene was digested… CONTINUE READING

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